Multiple transcription unit systemes, vectors and host cells...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01)

Patent

CA 2567251

The present invention provides a recombinant transcription unit capable of producing an RNA transcript of a predetermined size comprising a regulatory sequence operably linked to a nucleotide sequence comprising a transcribed region such that the transcription of said transcribed region is controlled by said regulatory sequence. The transcribed region comprises a region that encodes for a viral sequence, and a non-coding region downstream of the region encoding for said viral sequence, wherein the non-coding region comprises a nucleotide sequence encoding a cis-acting ribozyme. Methods of using the recombinant transcription unit, and cells containing vectors comprising the recombinant transcription unit are also disclosed.

La présente invention concerne une unité de transcription recombinée capable de produire un élément de transcription d'ARN de taille prédéterminée comprenant une séquence régulatoire éventuellement reliée à une séquence de nucléotides comprenant une zone transcrite de sorte que la transcription de ladite zone transcrite, est commandée par ladite séquence régulatoire. Le zone transcrite comprend un zone qui code pour une séquence virale, et une zone non codante en aval de la zone qui code pour ladite séquence virale, la zone non codante comprenant une séquence de nucléotides qui code pour une ribozyme à action cis. L'invention a également pour objet des procédés pour se servir de l'unité de transcription recombinée, et des cellules contenant des vecteurs qui comprennent l'unité de transcription recombinée.

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