Multiplex amplification and detection

Details Multiplex amplification and detection Multiplex amplification and detection

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2738792

The invention relates to the field of multiplex amplification. In particular, the invention relates to methods for assaying a sample for one or more nucleic acid targets in a single reaction based on the distinct melting temperatures or melting profiles of primers and/or probes. The invention also provides probes and kits for use in such methods.

L'invention concerne le domaine de l'amplification multiplex. En particulier, l'invention porte sur des procédés d'essai d'un échantillon pour une ou plusieurs cibles d'acide nucléique dans une seule réaction basée sur les températures de fusion distinctes ou les profils de fusion d'amorces et/ou de sondes. L'invention porte également sur des sondes et des coffrets destinés à être utilisés dans de tels procédés.

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