Multiplex amplification of short tandem repeat loci

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2352042

Methods and materials are disclosed for use in simultaneously amplifying at least thirteen loci of genomic DNA in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of at least thirteen short tandem repeat loci, including specific materials and methods for the analysis of thirteen such loci specifically selected by the United States Federal Bureau of Investigation as core loci for use in the Combined DNA Index System (CODIS) database.

Cette invention se rapporte à des procédés et à des matériels à utiliser dans l'amplification simultanée d'au moins treize loci d'ADN génomique dans une réaction multiplex simple, ainsi qu'à des procédés et à des matériels à utiliser dans l'analyse des produits de ces réactions. Sont également inclus dans cette invention des matériels et des procédés servant à l'amplification simultanée d'au moins treize loci à séquences répétées en tandem courtes, y compris des matériels et des procédés spécifiques pour l'analyse de treize de ces loci spécialement sélectionnés par le Federal Bureau of Investigation (FBI) des Etats-Unis comme loci noyaux à utiliser dans la base de données du système d'index d'ADN combinée (CODIS).

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