Multiplex assay methods and compositions

C - Chemistry – Metallurgy – 09 – K

Patent

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Details

C09K 11/07 (2006.01) C12Q 1/00 (2006.01) C40B 30/04 (2006.01) C40B 70/00 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2753269

Luminescence assays and compositions for assay of biomolecular interaction and activity and detection of modulators of biomolecular interaction and activity are provided. Technology described herein has utility in a variety of assay formats and types, for example, simultaneous monitoring multiple parameters which affect interaction and activity of biological molecules. Compositions and methods are provided herein which include a first solid-phase support associated with a first specific binding agent and a photosensitizer,; a second solid-phase support associated with a second specific binding agent and a first emission system; and a third solid-phase support associated with a third specific binding agent and a second emission system.

L'invention porte sur des essais par luminescence et sur des compositions pour l'évaluation d'une interaction et d'une activité biomoléculaire ainsi que la détection de modulateurs d'interaction et d'activité biomoléculaire. Selon l'invention, la technologie est utile dans une pluralité de formats et types d'essais, par exemple, la surveillance simultanée de multiples paramètres qui affecte l'interaction et l'activité de molécules biologiques. L'invention porte sur des compositions et des procédés qui comprennent un premier support en phase solide associé à un premier agent de liaison spécifique et à un photosensibilisant ; un second support en phase solide associé à un second agent de liaison spécifique et à un premier système d'émission ; et un troisième support en phase solide associé à un troisième agent de liaison spécifique et à un second système d'émission.

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