Multiplex branched-chain dna assays

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2607221

Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.

La présente invention concerne des procédés pour détecter au moins deux acides nucléiques dans le cadre d'un essai réalisé sur de l'ADN à chaîne ramifiée multiplexé. Différents acides nucléiques sont capturés par des événements d'hybridation coopérative sur différents sous-ensembles de particules identifiables ou en différents emplacements sélectionnés d'un support solide adressable spatialement. L'invention a également pour objet des compositions, des kits et des systèmes en relation avec ces procédés.

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