Multiplex genomic dna amplification for deletion detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

195/1.12, 150/8.

C12Q 1/68 (2006.01) C07K 14/47 (2006.01)

Patent

CA 1339731

The present invention relates to a method for detecting multiple DNA sequences simultaneously. The method involves amplification of multiple sequences simultaneously by annealing a plurality of paired oligonucleotide primers to single stranded DNA. One member of each pair is complementary to the sense strand of a sequences and the other member is complementary to a different segment of the anti-sense strand of the same sequence. The amplification occurs by alternately annealing and extending the primers. The invention also includes oligonucleotide primer sequences helpful in detecting genetic diseases and/or exogenous DNA sequences.

612823

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