Multiplex genotyping of populations of individuals

Details Multiplex genotyping of populations of individuals Multiplex genotyping of populations of individuals

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2297352

This invention provides methods for generating polymorphic profiles for many polymorphic markers in many individuals in a population. The methods involve performing multiplex amplification of the markers in many nucleic acid samples from each of many individuals to produce multiple amplification products. The resulting multiplex amplification products are applied to a substrate to create an array. Then, in one embodiment, in a series of iterative passes, pairs of probes that detect both alleles of a marker are hybridized to each amplification product in the array to identify the alleles the individuals have for the marker.

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