Multiplex pcr for detecting ehec infections

Details Multiplex pcr for detecting ehec infections Multiplex pcr for detecting ehec infections

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2393223

The invention relates to a method for detecting clinically relevant EHEC infections by amplifying both stxA1 and stxA2 sequences in a multiplex amplification reaction. The inventive method is further characterized in that both sequences of human-pathogenic and sequences of porcine-pathogenic stxA2 isoforms are amplified with the primers derived from a specific region of the stxA2 gene.

L'invention concerne un procédé permettant de mettre en évidence des infections à EHEC significatives sur le plan clinique, selon lequel une réaction d'amplification multiplex, ainsi que des séquences stx A1, de même que stx A2 sont amplifiées. Ce procédé se caractérise en ce que notamment, aussi bien des séquences d'isoformes stx A2 pathogènes chez l'homme que des séquences d'isoformes stx A2 pathogènes chez le porc, sont amplifiées avec des amorces issues d'une région déterminée du gène stx A2.

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