Multiplex protein fractionation

G - Physics – 01 – N

Patent

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G01N 33/541 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2645159

The invention provides methods, compositions, and kits for multiplex fractionation of proteins in a sample. Protein-binding molecules, such as small epitope antibodies or small epitope aptamers, are used for multiplex fractionation of proteins in a protein containing sample. Detection of fractionated proteins may be used for characterization of proteins in a sample in applications such as expression profiling, identification and/or quantification of proteins in a sample, and identification or detection of biomarkers.

L'invention concerne des procédés, des compositions, et des kits permettant le fractionnement multiplex de protéines dans un échantillon. Des molécules se liant à des protéines, telles que des anticorps se liant à de petits épitopes ou des aptamères se liant à de petits épitopes, sont utilisées en vue du fractionnement multiplex de protéines dans un échantillon contenant des protéines. La détection de protéines fractionnées peut être utilisée en vue de la caractérisation de protéines dans un échantillon dans des applications comme le profilage de l'expression, l'identification et/ou la quantification de protéines dans un échantillon, et l'identification ou la détection de biomarqueurs.

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