Multiplexed detection methods

Details Multiplexed detection methods Multiplexed detection methods

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2450139

The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.

La présente invention concerne des analyses multiplexées sensibles et précises pour la détection de mélanges à analyser cibles.

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