Multiply-primed amplification of nucleic acid sequences

Details Multiply-primed amplification of nucleic acid sequences Multiply-primed amplification of nucleic acid sequences

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2410951

Processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences. A kit containing components for use in the invention is also described.

L'invention concerne des procédés d'amplification de séquences d'ADN cibles se présentant sous la forme de molécules d'ADN circulaires simple ou double brin, notamment celles qui sont présentes dans les extraits de colonies et de plaques, lesquels procédés utilisent plusieurs amorces oligonucléotidiques de séquences spécifiques et/ou aléatoires. L'invention concerne également des méthodes de détection desdites séquences cibles amplifiées, ainsi qu'un kit renfermant les composants destinés à être utilisés dans le cadre de l'invention.

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