C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2669728
Embodiments of the invention herein described relate to multiplex polynucleotide sequence analysis without the use of size separation methods or blotting. In certain particulars the invention relates to multiplex sequencing using massively parallel sequencing methods, such as pyrosequencing methods and sequencing by synthesis. The invention provides increased throughput and the ability to analyze greater numbers of samples simultaneously or serially on presently available systems, as well as others yet to be developed. In certain of its embodiments the invention relates to the analysis of complex microbial communities, particularly to in-depth analysis thereof in large numbers of samples.
L'invention concerne l'analyse de séquences polynucléotidiques multiplexes sans l'utilisation de procédés de séparation de taille ou de transfert. Dans certains cas particuliers, l'invention concerne le séquençage multiplexe utilisant des procédés de séquençage en parallèle massivement, tels que des procédés de pyroséquençage et de séquençage par synthèse. L'invention fournit un débit augmenté et la capacité d'analyser un plus grand nombre d'échantillons simultanément ou séquentiellement sur des systèmes disponibles actuellement, ainsi que d'autres encore en développement. Dans certains de ses modes de réalisation, l'invention concerne l'analyse de communautés microbiennes complexes, en particulier leur analyse approfondie dans un grand nombre d'échantillons.
Biospherex Llc
Gowling Lafleur Henderson Llp
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