Multivalent hepatitis b virus vaccine

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/02 (2006.01) A61K 39/29 (2006.01) C07K 7/00 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C12N 7/04 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2078358

18345 TITLE OF THE INVENTION A MULTIVALENT HEPATITIS B VIRUS VACCINE ABSTRACT OF THE INVENTION In order to produce a mixture of different serotypes of hepatitis B virus (HBV) surface proteins in the form of particles, DNA encoding two or more HBV proteins were expressed by a single recombinant yea8t host. These HBV proteins display the antigenic sites genetically encoded by the S domain (including the preS domain) of the HBV virion envelope open reading frame and when expressed in a yeast host deficient in its ability to glycosylate, contain substantially reduced levels of entrapped carbohydrate when compared with HBsAg particles produced in yeast cells which are "wild-type" for glycosylation. These particles are useful as a vaccine for both the active and passive treatment or prevention of disease and/or infection caused by HBV or other agents serologically related to HBV including antigenic variants in the immunodominant epitopes of the surface protein, and are also uæeful as antigens and immunogens for development of diagnostic tests for such diseases or infections.

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