G - Physics – 01 – N
Patent
G - Physics
01
N
167/43
G01N 33/52 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1267081
ABSTRACT OF THE DISCLOSURE A multizone test device for the determination of analyte from a liquid test medium upon contact with the liquid test medium and a labeled reagent comprising a chemical group having a detectable physical property. The test device preferably com- prises multilayers including a reagent layer incor- porated with an immobilized reagent and a detection layer incorporated with an immobilized form of a binding substance for the labeled reagent. The im- mobilized reagent and the labeled reagent comprise specific binding partners which will bind to each other dependent upon the amount of analyte present. Labeled reagent which does not become bound to the immobilized reagent in the reagent layer migrates into the detection layer and becomes bound to and immobilized by the immobilized binding substance therein. As a result, reverse migration of the lab- eled reagent into the reagent layer is prevented and the detectable physical property provided by the label of the labeled reagent is localized in the de- tection layer for the precise measurement thereof and correlation to the amount of analyte in the test medium.
506751
Greenquist Alfred C.
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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