Murine expression of human ig.lambda. locus

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/13 (2006.01) A01K 67/027 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C12N 5/18 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2348898

In humans, approximately 60 % of expressed immunoglobulin light chains are of the Kappa type and 40 % of the Lambda type. In mice, there is almost no Lambda locus and over 95 % of light chains are of Kappa type. The present invention relates to transgenic mice carrying most of the human Ig Lambda light chain locus in their genome. The resulting mice express light chains with Kappa/Lambda ratio simlliar to the human ratio. Breeding of HulgL mice to Kappa-deficient mice is also described.

Chez l'homme, 60 % environ des chaînes légères exprimées de l'immunoglobuline sont du type Kappa et 40 % sont du type Lambda. Chez la souris, il n'existe pratiquement pas de locus Lambda et plus de 95 % des chaînes légères sont du type Kappa. La présente invention se rapporte à des souris transgéniques porteuses de la majorité du locus des chaînes légères Lambda de l'immunoglobuline humaine Ig dans leur génome. Les souris produites expriment les chaînes légères avec un rapport Kappa/Lambda similaire au rapport correspondant chez l'homme. L'invention se rapporte également à l'accouplement de souris HuIgL à des souris présentant une déficience en chaînes Kappa.

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