Muscle-specific expression vectors

Details Muscle-specific expression vectors Muscle-specific expression vectors

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) A01N 63/00 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2448120

The invention is directed to novel combinations of muscle-specifi c enhancer and promoter elements useful for achieving persistent expression in the muscle or myocytes. The muscle-specific promoter elements are derived from a muscle creatine kinase promoter, a troponin I promoter, a skeletal alpha-actin promoter, or a desmin promoter. The muscle-specific enhancer elements are derived from either troponin I internal regulatory elements, muscle creatin e kinase enhancers, or desmin enhancers.

L'invention concerne de nouvelles combinaisons d'éléments activateurs et promoteurs spécifiques aux muscles utilisés dans l'expression constante des muscles ou des myocytes. Les éléments promoteurs spécifiques aux muscles sont dérivés d'un promoteur de la créatine kinase musculaire, d'un promoteur de troponine I, d'un promoteur d'alpha-actine du squelette, ou d'un promoteur de desmine. Les éléments activateurs spécifiques aux muscles sont dérivés d'éléments régulateurs internes de troponine I, d'activateurs de la créatine kinase musculaire, ou d'activateurs de desmine.

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