C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12P 7/18 (2006.01)
Patent
CA 2373312
The present invention relates to mutant 1,3-propanediol dehydrogenase and a novel microorganism that is capable of growing in concentrations of at least 105 g/l 1,3-propanediol, levels normally toxic to wild-type microorganisms. The present invention also provides expression vectors and host cells comprising the mutant 1,3-propanediol dehydrogenase as well as methods for producing 1,3-propanediol comprising the use of cells comprising the mutant 1,3-propanediol dehydrogenase.
L'invention concerne la 1,3-propanediol déshydrogénase mutante et un nouveau microorganisme capable de se développer dans des concentrations d'au moins 105g/l de 1,3-propanediol, des niveaux habituellement toxiques pour les microorganismes de type sauvage. L'invention concerne également des vecteurs d'expression et des cellules hôtes renfermant la 1,3-propanediol déshydrogénase mutante, ainsi que des méthodes de préparation du 1,3-propanediol consistant à utiliser les cellules renfermant la 1,3-propanediol déshydrogénase mutante.
Selifonova Olga V.
Trimbur Donald E.
Whited Gregory M.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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