C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C07K 14/765 (2006.01) C12N 1/19 (2006.01) C12N 9/26 (2006.01) C12N 15/14 (2006.01)
Patent
CA 2063890
An object of the invention is to provide a novel promoter for high-level production of heterologous proteins and a method of obtaining the promoter. Thus, a mutant AOX2 promoter is derived from the natural AOX2 promoter by base sequence deletion, base substitution or insertion. A microbial strain carrying the AOX2 gene under the control of such a mutant AOX2 promoter can be obtained by cultivating a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter in a methanol-containing medium. A heterologous protein can be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from a promoter. Furthermore, a plasmid carrying the mutant AOX2 promoter is provided. A heterologous protein can be produced by using a transformant obtained by incorporation of such plasmid.
Ishida Yutaka
Kawabe Haruhide
Miura Masami
Murakami Koji
Nakagawa Yukimitsu
Mitsubishi Pharma Corporation
Riches Mckenzie & Herbert Llp
The Green Cross Corporation
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