Mutant bacterium clostridium histolyticum, a process for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12N 9/52 (2006.01)

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CA 2047306

ABSTRACT A novel mutant of bacterium Clostridium histolyticum, designated Clostridium histolyticum K-26 cl? 88, of the folowing characteristics: - the lack of a motility system, which is a fundamental characteristic of the parenteral strain, and - the inability to biosynthesize the accompanying enzyme clostripain; it was deposited: i) at the Institute of Biochemical Engineering, Laboratory of Microbiology, Zagreb, Pierottijeva 6, on May 22, 1990, under the accession number 4043, ii) and in conformance with the Budapest Treaty on the International Recognition of the Deposit of Micro- organisms for the Purposes of Patent Procedure, pursuant to Rule 6.1, at the National Collection of Agricultural and Industrial Microorganisms, Department of Microbiology and Biotechnology, University of Horticulture and Food Industry , Somloi ut 14-16, H-1118 Budapest, Hungary on June 4, 1991, under the accession number NCAIM B (P) 001145; further, a process of obtaining said novel mutant and its use in a process for the production of clostripain-free collagenase.

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