C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2467084
Nucleic acid compositions encoding mutants of wild-type chromo/fluoroproteins whose chromo/fluorescent properties have been interconverted, as well as the proteins encoded the same, are provided. Also provided are methods for interconverting chromoproteins to fluorescent proteins, and vice versa. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
L'invention concerne des compositions d'acides nucléiques codant des mutants de chromo/fluoroprotéines de type sauvage dont les propriétés chromo/fluorescentes ont été interconverties, ainsi que des protéines codées de la même manière. L'invention concerne également des procédés permettant d'interconvertir des chromoprotéines en protéines fluorescentes, et vice versa. L'invention concerne également des protéines sensiblement analogues aux protéines spécifiques mentionnées ci-dessus ou des mutants de ces protéines. L'invention concerne des fragments d'acides nucléiques et les peptides codés par ces fragments, ainsi que des anticorps de ces protéines, des cellules transgéniques et des organismes. La protéine de l'invention et des compositions d'acides nucléiques peuvent être utilisées dans une variété d'applications différentes. Enfin, des trousses à utiliser dans de telles applications, par exemple comprenant les compositions d'acides nucléiques mentionnées ci-dessus, sont décrites.
Bulina Maria E.
Chudakov Dmitry
Lukyanov Konstantin Anatolievich
Clontech Laboratories Inc.
Smart & Biggar
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