Mutant .delta.8 desaturase genes engineered by targeted...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/12 (2006.01)

Patent

CA 2669217

The present invention relates to mutant .DELTA.8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 .omega.-6, EDA] to dihomo-.gamma.-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 .omega.-3, ETrA] to eicosatetraenoic acid [20:3 .omega.-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant .DELTA.8 desaturases in plants and oleaginous yeast are disclosed.

La présente invention concerne des gènes mutants de la désaturase delta 8, qui ont la capacité de transformer l'acide eicosadiènoïque [20:2 .omega.-6, EDA] en acide dihomo-.gamma.-linolénique [20:3, DGLA] et/ou l'acide eicosatriénoïque [20:3 .omega.-3, ETrA] en acide eicosatetraénoïque [20:3 .omega.-3, ETA]. L'invention concerne aussi des fragments d'acides nucléiques isolés et des construit de recombinaison comprenant ces fragments codant pour la désaturase delta huit ainsi que des procédés de fabrication d'acides gras polyinsaturés de longues chaînes (PUFA) utilisant ces désaturases delta huit dans des plantes et des levures oléagineuses.

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