Mutant dna polymerases and related methods

Details Mutant dna polymerases and related methods Mutant dna polymerases and related methods

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01)

Patent

CA 2666758

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

L'invention porte sur des ADN polymérases mutantes possédant des vitesses d'extension améliorées par rapport à la polymérase non modifiée correspondante. Les polymérases mutantes précitées sont utilisées dans une variété de procédés d'extension d'amorce. L'invention concerne également des compositions associées, y compris des acides nucléiques recombinants, des vecteurs et des cellules hôtes qui sont utilisés, p.ex., dans la production des ADN polymérases mutantes.

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