C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2376056
The present invention relates to a method for producing mutants of a fatty acid desaturase, the unmutagenized pre-cursor having 18-carbon chain substrate specificity, the produced mutants having substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons, relative to the activity of unmutagenized precursor desaturase. DNA en-coding the mutants, host cells containing the DNA, transgenic plants expressing the DNA and the desaturase enzyme produced by the method are also recited.
La présente invention concerne un procédé destiné à produire des mutants d'une désaturase d'acides gras, la désaturase précurseur non mutagénisée possédant une spécificité par rapport à un substrat comportant une chaîne de 18 atomes de carbone, les mutants produits présentant une activité sensiblement améliorée, par rapport à celle de la désaturase précurseur non mutagénisée, sur les substrats d'acides gras dont les chaînes contiennent moins de 18 atomes de carbone. L'invention concerne également l'ADN codant les mutants, les cellules hôtes contenant l'ADN, les plantes transgéniques exprimant l'ADN et l'enzyme désaturase produite par le procédé.
Brookhaven Science Associates Llc
Goudreau Gage Dubuc
LandOfFree
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