Mutant microbial .alpha.-amylases with increased thermal,...

Details Mutant microbial .alpha.-amylases with increased thermal,... Mutant microbial .alpha.-amylases with increased thermal,...

C - Chemistry, Metallurgy

12

N

195/39, 195/60,

C12N 15/56 (2006.01) C08B 30/12 (2006.01) C12N 9/26 (2006.01) C12N 9/28 (2006.01) C12N 9/30 (2006.01) C12N 9/32 (2006.01) C12N 15/70 (2006.01) C12N 15/75 (2006.01) D06L 1/06 (2006.01)

Patent

CA 2030554

Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.- amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.

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