Mutant phosphoenolpyruvate carboxylase, its gene, and...

Details Mutant phosphoenolpyruvate carboxylase, its gene, and... Mutant phosphoenolpyruvate carboxylase, its gene, and...

C - Chemistry, Metallurgy

12

N

C12N 9/88 (2006.01) C12N 15/60 (2006.01) C12P 13/04 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01) C12P 13/14 (2006.01) C12P 13/20 (2006.01)

Patent

CA 2169170

A phosphoenolpyruvate carboxylase gene, which has mutation such as mutation to replace 625th glutamic acid from the N-terminus of phosphoenolpyruvate carboxylase with lysine, mutation to replace 438th arginine from the N-terminus with cysteine and the like, is introduced into Escherichia coli or coryneform bacteria, so as to produce a phosphoenolpyruvate carboxylase which is not substantially inhibited by aspartic acid, thereby amino acid is efficiently produced.

Cet allèle de phosphénolpyruvate carboxylase, qui n'est pas sensiblement inhibé par l'acide aspartique, est produit en introduisant dans l'Escherichia coli (ou dans une bactérie corynéiforme) un gène allèle de phosphénolpyruvate carboxylase. Ce gène peut être du type de ceux dans lesquels le résidu 625 de l'acide glutamique provenant de l'extrémité N de la carboxylase a été remplacé par un résidu de lysine ou du type de ceux dans lesquels le résidu 438 de l'arginine a été remplacé par un résidu de cystéine. Cette carboxylase présente une bonne efficacité dans la production d'un acide aminé.

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