Mutant pneumolysin proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/09 (2006.01) C07K 14/315 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2566344

The invention relates to immunogenic compositions comprising mutant Streptococcus pneumoniae pneumolysin proteins. The invention further relates to such proteins and nucleic acids encoding these proteins. In particular embodiments, the invention is directed to an isolated mutant pneumolysin (PLY) protein, wherein the mutant PLY protein differs from the wild type PLY protein by the presence of a mutation within the region of amino acids 144 to 161 of the wild type sequence, such that the toxicity of the mutant is reduced relative to that of the wild-type protein. In particular embodiments, the mutant PLY protein differs from the wild type protein by the substitution or deletion of amino acids within this region, including the deletion of two adjacent amino acids within the region of amino acids 144 to 151 of the wild type sequence.

La présente invention concerne des compositions immunogènes comprenant des protéines de pneumolysine Streptococcus pneumoniae mutantes. Elle concerne également de telles protéines et des acides nucléiques codant ces protéines. Dans des modes de réalisation particuliers, cette invention concerne une protéine de pneumolysine mutante isolée (PLY) qui diffère de la protéine PLY de type sauvage de par la présence d'une mutation dans la région des acides aminés 144 à 161 de la séquence du type sauvage, de manière que la toxicité de la protéine mutante est réduite par rapport à celle de la protéine de type sauvage. Dans des modes de réalisation particuliers, la protéine PLY mutante diffère de la protéine de type sauvage de par la substitution ou la délétion d'acides aminés dans cette région, notamment de par la délétion de deux acides aminés adjacents dans la région des acides aminés 144 à 151 de la séquence du type sauvage.

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