Mutant polymerases for sequencing and genotyping

Details Mutant polymerases for sequencing and genotyping Mutant polymerases for sequencing and genotyping

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01)

Patent

CA 2581471

The invention relates to the discovery of novel mutant DNA polymerases that possess altered kinetics for incorporating phosphate-labeled nucleotides during polymerization. The invention further relates to the use of these mutant DNA polymerases in sequencing and genotyping methods.

La présente invention a trait à la découverte de nouvelles polymérases d'ADN mutantes qui présentent une cinétique modifiée pour l'incorporation de nucléotides à marqueurs phosphate lors de la polymérisation. L'invention a également trait à l'utilisation de ces polymérases d'ADN mutantes dans des procédés de séquençage et de génotypage.

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