Mutant strains capable of producing chemically diversified...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/00 (2006.01) A61K 38/16 (2006.01) C12N 1/20 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2407595

The invention concerns mutant prokaryotic cells, in particularE.coli, capable of producing proteins whereof the amino acid sequences comprise at least a non-conventional amino acid, methods for producing and purifying said proteins and the proteins obtained by said methods. The invention also concerns uses of said cells and proteins in different fields such as in therapy, cosmetics, diagnosis or biosynthesis or biodegradation of organic compounds.

La présente invention a pour objet des cellules procaryotes mutantes, notamment E.coli, capables de produire des protéines dont les séquences d'acides aminés comprennent au moins un acide aminé non conventionnel, des procédés de production et de purification desdites protéines ainsi que les protéines obtenues par les procédés selon l'invention. L'invention comprend également les applications desdites cellules et protéines dans différents domaines tels que le domaine thérapeutique, cosmétique, diagnostic ou de la biosynthèse ou la biodégradation de composé organique.

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