Mutant tn5 transposase enzymes and method for their use

Details Mutant tn5 transposase enzymes and method for their use Mutant tn5 transposase enzymes and method for their use

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2380850

Modified Tn5 transposase proteins having a preference for transposon Tn5 inside ends rather than outside ends are disclosed and can be use in combination with transposase enzymes that prefer outside ends to inside ends in a method for end-specific directed transposition <i>in vivo</i> or <i>in vitro</i>.

L'invention se rapporte à des protéines Tn5 transposases modifiées possédant une préférence pour les extrémités internes plutôt que pour les externes du transposon Tn5. Ces protéines peuvent être utilisées en association à des enzymes transposases qui préfèrent les extrémités externes aux extrémités internes lors de la mise en oeuvre d'un procédé de transposition directe <i>in vitro</i> ou <i>in vivo</i>, spécifique de certaines extrémités.

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