C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C07K 14/195 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2419453
The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
L'invention porte sur un procédé de conception et construction d'une variante de transglutaminase provenant du <i>Streptoverticillium mobaraense</i> et basé sur la stéréostructure de la MTG, et sur la variante de la MTG ainsi réalisée. On obtient ainsi avec ledit procédé une transglutaminase améliorée en ce qui concerne sa réactivité vis-à-vis du substrat. Dans ledit procédé la variante de transglutaminase s'obtient par estimation du site de fixation de la MTG au substrat en partant d'une stéréostructure obtenue par des rayons X, puis en analysant la structure cristalline des cristaux de MTG, puis en substituant et ou supprimant le résidu d'acide aminé situé sur le site de fixation de la transglutaminase au substrat.
Hirayama Kazuo
Ishikawa Kohki
Kashiwagi Tatsuki
Shimba Nobuhisa
Suzuki Ei-Ichiro
Ajinomoto Co. Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
LandOfFree
Mutant transglutaminase from streptoverticillium having... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mutant transglutaminase from streptoverticillium having..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutant transglutaminase from streptoverticillium having... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1874347