Mutated photoproteins and their uses

Details Mutated photoproteins and their uses Mutated photoproteins and their uses

C - Chemistry, Metallurgy

07

K

C07K 14/435 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2455542

The invention concerns mutated photoproteins derived from isolated jellyfish photoproteins, said mutated photoproteins being characterised in that they exhibit a thermostability higher than that of the photoproteins from which they are derived, and are called thermostable mutated photoproteins, and/or a luminescence duration longer than that of the photoproteins from which they are derived, and are called persistent mutated photoproteins. The invention also concerns the use of said mutated photoproteins in methods for detecting in vitro molecules in a biological sample, methods for detecting compounds with enzymatic activity in a biological sample, or methods for detecting intracellular calcium variations.

L'invention a pour objet des photoprotéines mutées dérivées des photoprotéines isolées de méduses, lesdites photoprotéines mutées étant caractérisées par une thermostabilité superieure à celle des photoprotéines dont elles dérivent, et sont désignées photoprotéines mutées thermostables, et/ou par un temps de luminescence supérieur à celui des photoprotéines dont elles dérivent, et sont désignées photoprotéines mutées persistantes. L'invention concerne également l'utilisation des photoprotéines mutées susmentionnées dans le cadre de la mise en oeuvre de procédés de détection in vitro de molécules dans un échantillon biologique, de procédés de detection de composes à activité enzymatique dans un échantillon biologique, ou de procédés de détection des variations de calcium intracellulaire.

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