Mutation detecting method using photobridging-stabilized...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2128418

2128418 9315223 PCTABS00163 L'invention concerne un procédé de détection de mutations par électrophorèse d'ADN double brin en gradient de dénaturation, caractérisé en ce que l'hybride analysé est stabilisé par photopontage au moyen d'un ou plusieurs agents intercalants photoactivés. Selon le procédé de l'invention, l'hybride analysé est obtenu par un processus d'amplification génétique d'un fragment d'ADN double brin, dont les phases d'hybridation sont effectuées avec deux oligonucléotides amorces sur l'un desquels au moins se trouve fixé un ou plusieurs agents intercalants photoactivables.

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