Mutation of dna polymerases from archaeobacteria

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 15/03 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2475418

The present invention relates to a variant archaeal DNA polymerase having a modified amino acid sequence of a wild-type amino acid sequence, the modified sequence being in the amino-terminal amino acids that comprise a uracil- binding pocket in the wild-type polymerase whereby the variant polymerase has reduced affinity for uracil than the wild-type polymerase. Such variant polymerases may be usefully employed in biological assay systems such as the polymerase chain reaction.

Variante de l'ADN polymérase d'archéobactéries qui possède une séquence d'acides aminés modifiée par rapport à une séquence d'acides aminés de type sauvage, ladite séquence se trouvant dans les acides aminés amino-terminaux qui comprennent une poche de liaison d'uracile dans la polymérase de type sauvage. Cette variante de polymérase possède une affinité réduite pour l'uracile par rapport à la polymérase de type sauvage. Ces variantes de polymérase peuvent être utilement employées dans des systèmes d'analyse biologique, tels que dans la réaction d'amplification en chaîne par polymérase.

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