Mycobacteria virulence factors and a method for their...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/04 (2006.01) C07K 14/35 (2006.01) C12N 15/74 (2006.01) C12Q 1/68 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2179772

The present invention provides polynucleotides associated with virulence in mycobacteria, and particularly a fragment of DNA isolated from M. bovis that contains a region encoding a putative sigma factor. Also provided are methods for a DNA sequence or sequences associated with virulence determinants in mycobacteria, and particularly in M. tuberculosis and M. bovis. The invention also provides corresponding polynucleotides associated with avirulence in mycobacteria. In addition, the invention provides a method for producing strains with altered virulence or other properties which can themselves be used to identify and manipulate individual genes.

L'invention concerne des polynucléotides associés à la virulence dans des mycobactéries, et plus particulièrement un fragment d'ADN isolé à partir de M. bovis qui contient une région codant un facteur sigma putatif. L'invention porte également sur des procédés destinés à une séquence d'ADN ou à des séquences associées aux déterminants de virulence dans des mycobactéries, et plus particulièrement dans M. tuberculosis et M. bovis. De plus, l'invention se rapporte aux polynucléotides correspondants associés à l'avirulence dans des mycobactéries, et à un procédé de production de souches présentant une virulence modifiée ou d'autres propriétés pouvant être utilisées pour identifier et manipuler des gènes individuels.

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