Mycobacterial antigens expressed during latency

Details Mycobacterial antigens expressed during latency Mycobacterial antigens expressed during latency

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 31/7088 (2006.01) A61K 35/74 (2006.01) A61K 38/16 (2006.01) A61K 39/04 (2006.01) A61K 39/395 (2006.01) C07H 21/00 (2006.01) C07K 14/35 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/74 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2453173

A method is provided for identifying mycobacterial genes that are induced or up-regulated under culture conditions that are nutrient-starving and which maintain mycobacterial latency, said conditions being obtainable by batch fermentation of a mycobacterium for at least 20 days post-inoculation, when compared with culture conditions that are not nutrient-starving and which support exponential growth of said mycobacterium. Said induced or up-regulated genes form the basis of nucleic acid vaccines, or provide targets to allow preparation of attenuated mycobacteria for vaccines against mycobacterial infections. Similarly, peptides encoded by said induced or up-regulated genes are employed in vaccines. In a further embodiment, the identified genes/peptides provide the means for identifying the presence of a mycobacterial infection in a clinical sample by nucleic acid probe or antibody detection.

L'invention concerne un procédé permettant d'identifier des gènes mycobactériens qui sont induits ou activés dans des conditions de culture caractérisés par un sous-apport d'éléments nutritifs, et qui permettent de préserver la latence mycobactérienne, ces conditions étant obtenues par une fermentation discontinue d'une mycobactérie pendant au moins 20 jours après l'inoculation, à la différence des conditions de cultures ne comprenant pas de sous-nutrition et qui produisent une multiplication exponentielle de ladite mycobactérie. Lesdits gènes induits ou activés constituent la base de vaccins d'acide nucléique, ou peuvent fournir des cibles pour la préparation de mycobactéries atténuées destinées à des vaccins contre les infections mycobactériennes. De manière similaire, les peptides codés par ces gènes induits ou activés sont utilisés dans des vaccins. Dans un mode de réalisation différent, les gènes/peptides permettent l'identification de la présence d'une infection mycobactérienne dans un échantillon clinique, au moyen d'une sonde d'acide nucléique ou de la détection d'anticorps.

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