Mycobacterial species-specific reporter mycobacteriophages

Details Mycobacterial species-specific reporter mycobacteriophages Mycobacterial species-specific reporter mycobacteriophages

C - Chemistry, Metallurgy

12

N

C12N 7/01 (2006.01) C07K 14/01 (2006.01) C07K 14/35 (2006.01) C12N 7/00 (2006.01) C12N 15/74 (2006.01) C12Q 1/18 (2006.01) C12Q 1/34 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2129034

2129034 9316172 PCTABS00025 This invention relates to mycobacterial species-specific reporter mycobacteriophages (reporter mycobacteriophages), methods of producing such reporter mycobacteriophages and the use of such reporter mycobacteriophages for the rapid diagnosis of mycobacterial infection and the assessment of drug susceptibilities of mycobacterial strains in clinical samples. In particular, this invention is directed to the production and use of luciferase reporter mycobacteriophages to diagnose tuberculosis. The mycobacterial species-specific reporter mycobacteriophages comprise mycobacterial species-specific mycobacteriophages which contain reporter genes and transcriptional promoters therein. When the reporter mycobacteriophages are incubated with clinical samples which may contain the mycobacteria of interest, the gene product of the reporter genes will be expressed if the sample contains the mycobacteria of interest, thereby diagnosing mycobacterial infection.

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