Myeloma cell culture in transferrin-free low iron medium

Details Myeloma cell culture in transferrin-free low iron medium Myeloma cell culture in transferrin-free low iron medium

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12N 5/02 (2006.01)

Patent

CA 2534286

The present invention relates to a method for culturing mammalian cells in a culture medium which is transferrin free and which contains no lipophilic or synthetic nitrogen-containing chelators. Also provided is the use of the medium and a process for providing a mammalian product by culturing cells capable of producing the product in the medium.

L'invention concerne un procédé de mise en culture de cellules mammaliennes dans un milieu de culture exempt de transferrine et dépourvu de chélateur lipophile ou de chéalteur de synthèse contenant de l'azote. Cette invention se rapporte également à l'utilisation dudit milieu, ainsi qu'à un procédé d'obtention d'un produit mammalien par mise en culture de cellules pouvant produire ledit produit dans le milieu.

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