Myoglobin with peroxidase activity

Details Myoglobin with peroxidase activity Myoglobin with peroxidase activity

C - Chemistry, Metallurgy

07

K

C07K 14/805 (2006.01) C07K 14/47 (2006.01) C12P 1/00 (2006.01) C12P 3/00 (2006.01) C12P 9/00 (2006.01)

Patent

CA 2185605

Myoglobin is shown to have Manganese (Mn2+) binding and peroxidase capacity. Mn binding and peroxidase activity is enhanced by modification of the amino acidsequence of myoglobin to provide a Mn binding site on the surface of the protein near the heme group. Peroxidase activity of myoglobin not specific to Mn is enhanced by substituting amino acids at residues 39, 45, 46, 97 and 107 of myoglobin.

L'invention concerne une myoglobine qui possède une capacité de fixation du manganèse (Mn2+) et une activité peroxydasique. L'activité de fixation du Mn et peroxydasique est accrue par la modification de la séquence d'acides aminés de la myoglobine pour incorporer un site de fixation du Mn à la surface de la protéine près du groupe hème. L'activité peroxydasique de la myoglobine non spécifique du Mn est augmentée par substitution des acides aminés dans les résidus 39, 45, 46, 97 et 107 de la myoglobine.

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