N-substituted color-forming couplers and their use in the...

Details N-substituted color-forming couplers and their use in the... N-substituted color-forming couplers and their use in the...

C - Chemistry, Metallurgy

07

F

150/2, 260/283,

C07F 9/60 (2006.01) C12Q 1/28 (2006.01) C12Q 1/54 (2006.01) C12Q 1/62 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1258857

-0- N-SUBSTITUTED COLOR-FORMING COUPLERS AND THEIR USE IN THE ANALYTICAL DETERMINATION OF HYDROGEN PEROXIDE OR OTHER ANALYTES Abstract of the Disclosure Particular heterocyclic compounds are useful as color-forming couplers in analytical compositions, elements and methods. These compounds are represented by the structure: Image wherein R is a solubilizing group, R1, R2, R3 and R4 are independently hydrogen, alkyl, alkoxy, aryl, aryloxy, a heterocyclic group, halo, amino or R, and Z represents the carbon atoms necessary to complete the nucleus of a 5- or 6-membered fused ring. The color-forming coupler is combined with an oxidizable color developing compound, e.g. 4-amino- antipyrine to provide a chromogenic composition. This composition can be used in solution or dry assays of biological fluids to produce a color dye in the presence of hydrogen peroxide or other analytes, e.g. glucose or uric acid, which react to produce hydrogen peroxide.

473863

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