C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/12 (2006.01) C07C 227/18 (2006.01) C07C 229/08 (2006.01) C07C 229/24 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2194446
Novel alkoxythiocarbonylimidazoles provide new reagents for the N-terminal sequencing of small polypeptides samples. These reagents form an alkoxy thiourea derivative which is cleaved with acid to remove the N-terminal amino acid as a stable thiazolinone which does not rearrange to a thiohydantoin. This thiazolinone may be derivatized to provide a detectable group such as a fluorescent group or an ionizable group detectable by mass spectrometry.
De nouvelles alcoxythiocarbonylimidazoles constituent de nouveaux réactifs utiles pour le séquençage N-terminal de petits échantillons de polypeptides. Ces réactifs forment un dérivé alcoxy thiourée qui est clivé par un acide pour extraire l'acide aminé N-terminal tel qu'une thiazolinone stable qui ne subit pas de réarrangement en une thiohydantoïne. On peut transformer en dérivé cette thiazolinone afin de produire un groupe détectable tel qu'un groupe fluorescent ou un groupe ionisable, détectable par spectrométrie de masse.
City Of Hope
Osler Hoskin & Harcourt Llp
LandOfFree
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