C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/87 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07H 17/00 (2006.01) C07H 21/00 (2006.01) C07K 19/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2693525
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
La présente invention a pour objet un nouveau procédé pour la transduction et/ou la transfection de cellules végétales. Des peptides de pénétration cellulaire (CPP) ont été employés avec succès en tant que nanosupports pour délivrer des protéines et des oligonucléotides à des microspores de cellule végétale simple ainsi qu'à des embryons zygotiques multicellulaires. L'efficacité de l'internalisation des CPP et en outre de la délivrance d'un chargement macromoléculaire comprenant une protéine et/ou un oligonucléotide peut être amplifiée par la perméabilisation des embryons zygotiques.
Chugh Archana
Eudes Francois
Agriculture And Agri-Food Canada
Gowling Lafleur Henderson Llp
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