Nanoliter-scale synthesis of arrayed biomaterials and...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/02 (2006.01)

Patent

CA 2539670

A population of embryonic epithelial cells produced in vitro from embryonic stem cells. In one embodiment, at least 45% of the cells express cytokeratin, for example, cytokeratin-7. A method of screening cell-polymer interactions. The method includes depositing monomers as a plurality of discrete elements on a substrate, causing the deposited monomers to polymerize, thereby creating an array of discrete polymer elements on the substrate, incubating the substrate in a cell-containing culture medium, and characterizing a predetermined cell behavior on each polymer element.

Une population de cellules épithéliales embryonnaires est produite in vitro à partir de cellules souches embryonnaires. Dans une forme d'exécution, au moins 45 % des cellules expriment de la cytokératine, par exemple, de la cytokératine-7. L'invention concerne un procédé de criblage d'interactions cellule-polymère, procédé comprenant : le dépôt de monomères, sous forme d'une pluralité d'éléments discrets, sur un substrat, provoquant ainsi la polymérisation des monomères déposés, et créant ainsi un réseau d'éléments polymères discrets sur le substrat ; l'incubation du substrat dans un milieu de culture contenant des cellules ; et la caractérisation d'un comportement cellulaire prédéterminé sur chaque élément polymère.

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