Nanoparticles comprising biologically active tnf which is...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 17/00 (2006.01) A61K 38/19 (2006.01) A61K 47/48 (2006.01) C07K 14/52 (2006.01) C07K 17/06 (2006.01)

Patent

CA 2458682

The invention relates to nanoparticles comprising tumour necrosis factor (TNF) or cytokine, immobilised thereon, the TNF or cytokine preferably being oriented in the form of a trimer and being immobilised on the carrier while maintaining its biological activity. The invention also relates to methods for the oriented immobilisation of TNF or cytokine on nanoparticles, to the use of nanoparticles comprising immobilised TNF or cytokine for the identification and/or isolation of cytokine or TNF binding partners, and for the identification and/or isolation of inhibitors of the interaction between TNF or cytokine and the binding partners thereof, to the use of such nanoparticles for producing a pharmaceutical composition, especially for the treatment of tumours, and to pharmaceutical and diagnostic compositions containing such nanoparticles.

L'invention concerne des nanoparticules comportant le facteur de nécrose des tumeurs (TNF) ou de la cytokine, immobilisé(e) dessus, le TNF ou la cytokine étant orienté(e) de préférence sous forme de trimère et étant immobilisé(e) sur le support, tout en conservant son activité biologique. L'invention concerne également des procédés d'immobilisation orientée de TNF ou de cytokine sur des nanoparticules, l'utilisation des nanoparticules présentant le TNF ou de la cytokine immobilisé(e), pour identifier et/ou isoler des partenaires de liaison de la cytokine ou du TNF, ainsi que pour identifier et/ou isoler des inhibiteurs de l'interaction entre le TNF ou la cytokine et ses partenaires de liaison, l'utilisation de ce type de nanoparticules pour produire une composition pharmaceutique, s'utilisant notamment dans le traitement de tumeurs, ainsi que des compositions pharmaceutiques et diagnostiques contenant ce type de nanoparticules.

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