Nanoparticles having oligonucleotides attached thereto and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07B 61/00 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2402955

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

L'invention concerne des procédés de détection d'un acide nucléique. Ces procédés consistent à mettre en contact l'acide nucléique avec au moins un type de particules auxquelles sont attachés des oligonucléotides. Dans un mode de réalisation de l'invention, les oligonucléotides sont attachés à des nanoparticules et ont des séquences complémentaires à des parties de la séquence de l'acide nucléique. Un changement détectable (de préférence un changement de couleur) résulte de l'hybridation des oligonucléotides sur nanoparticules avec l'acide nucléique. L'invention concerne également des compositions et des kits contenant des particules. L'invention concerne encore des procédés de synthèse des conjugués nanoparticules-oligonucléotides uniques, les conjugués ainsi produits et les procédés d'utilisation de ces conjugués. En plus, l'invention concerne des nanomatières et nanostructures comprenant les nanoparticules et des procédés de nanoproduction utilisant des nanoparticules. L'invention concerne enfin un procédé de séparation d'un acide nucléique sélectionné parmi les autres acides nucléiques.

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