Nanoparticles having oligonucleotides attached thereto and...

Details Nanoparticles having oligonucleotides attached thereto and... Nanoparticles having oligonucleotides attached thereto and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) B82B 1/00 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2376623

The invention provides methods and kits for detecting nucleic acids. The methods employ particles and substrates having oligonucleotides attached to them. The presence of a nucleic acid produces a detectable change, such as a change in color, conductivity, or optical density. The invention further provides a method of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the method, kits comprising the conjugates, nanomaterials and nanostructures comprising the conjugates, and methods of using the conjugates.

La présente invention concerne des procédés et des équipements de détection d'acides nucléiques. Ces procédés font intervenir des particules et des substrats auxquels sont rattachés des oligonucléotides. La présence d'un acide nucléique produit un changement détectable, tel qu'un changement de couleur, de conductivité ou de densité optique. Cette invention concerne également un procédé de synthèse de conjugués uniques nanoparticules-oligonucléotides, les conjugués obtenus par ce procédé, des équipements comprenant ces conjugués, des nanomatériaux et nanostructures comprenant ces conjugués, et des procédés d'utilisation de ces conjugués.

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