Nanoparticles having oligonucleotides attached thereto and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 1/06 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2262018

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing the nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

Procédé de détection d'un acide nucléique, qui consiste à mettre en contact ledit acide nucléique avec un ou plusieurs types de particules sur lesquelles sont attachés des oligonucléotides. Dans un mode de réalisation de la présente invention, les oligonucléotides sont attachés aux nanoparticules et possèdent des séquences complémentaires de parties de la séquence de l'acide nucléique. Un changement détectable (de préférence un changement de couleur) se produit en résultat de l'hybridation des oligonucléotides sur les nanoparticules à l'acide nucléique. La présente invention concerne également des nanomatériaux et des nanostructures comportant des nanoparticules et des procédés de nanofabrication faisant appel auxdites nanoparticules. Enfin, la présente invention concerne un procédé permettant de séparer un acide nucléique choisi d'autres acides nucléiques.

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