Native gp41 assay

G - Physics – 01 – N

Patent

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Details

G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2694278

The present invention concerns a method for identifying compounds that decrease the ability of Human Immunodeficiency Virus (HIV) to enter previously uninfected cells. More specifically it relates to a homogeneous competitive cell-based binding assay for the identification of HIV-1 entry fusion inhibitors on a high content screening platform or flow cytometric-based read out.

La présente invention concerne un procédé d'identification de composés capables de diminuer la capacité du virus de l'immunodéficience humaine (VIH) à pénétrer dans des cellules préalablement non infectées. Plus spécifiquement, la présente invention concerne un test compétitif homogène de liaison à base de cellules, qui permet l'identification d'inhibiteurs de fusion d'entrée HIV-1 sur une plate-forme d'essai à haute teneur ou un afficheur basé sur la cytométrie de flux.

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