Negative control media for microbiologic biochemical tests

C - Chemistry – Metallurgy – 12 – Q

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150/16.5

C12Q 1/20 (2006.01) C12M 1/20 (2006.01)

Patent

CA 1132444

53,436 CAN/JLG Abstract A microorganism identification test container comprises a tray member provided with a plurality of wells. Some of the wells are microorganism identification test wells having biochemical test media which permits growth of microorganisms with the generation of a volatile color-forming compound, Some of the wells are negative control wells including an inhibitor which prevents color formation in the negative control wells caused by the volatile color-forming compound generated in the test wells. Preferably a series of biochemical test media is adjacent to the corresponding negative control medium. Comparison between the negative control wells and the test wells, after inoculation and incubation of the microorganism, indicates the presence of a particular microorganism. Since many of the biochemical test media produce only subtle changes in response to microorganism activity, the appearance of the negative control must remain essentially unchanged or subtle changes in the biochemical test wells will not be perceived.

354119

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