Negatively charged membrane

B - Operations – Transporting – 01 – D

Patent

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Details

B01D 67/00 (2006.01) B01D 61/14 (2006.01) B01D 71/08 (2006.01) B01D 71/40 (2006.01) B01J 39/26 (2006.01) B01J 47/12 (2006.01) C07K 1/00 (2006.01) C07K 1/18 (2006.01) C07K 1/34 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2364839

The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N- (hydroxymethyl) - and/or N-(alkoxymethyl) - acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl) - or N-(alkoxymethyl) - acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.

La présente invention concerne, dans certains modes de réalisation, une membrane microporeuse chargée négativement comprenant un substrat et un film réticulé contenant des charges négatives fixées. Le film réticulé peut être préparé, par exemple, à partir d'une composition polymérisée comprenant un monomère non saturé ayant un groupe anionique, un N-(hydroxyméthyl) - et/ou N-(alkoxyméthyl) - acrylamide, un monomère non saturé hydrophilique et un initiateur. La présente invention concerne également, dans certains modes de réalisation, une membrane microporeuse chargée négativement comprenant un substrat poreux et un film réticulé préparé à partir d'une composition polymérisée comprenant un monomère non saturé ayant un groupe anionique, un N-(hydroxyméthyl) - ou N-(alkoxyméthyl) - acrylamide, un polysaccharide et un initiateur. Les membranes selon l'invention peuvent être utilisées dans la chromatographie d'échange d'ions, par exemple, dans la séparation et la purification d'espèces chimiques telles que les protéines.

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