Nested oligonucleotides containing hairpin for nucleic acid...

Details Nested oligonucleotides containing hairpin for nucleic acid... Nested oligonucleotides containing hairpin for nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2436693

Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library a polypeptides or proteins, such as, for example, an antibody library.

L'invention concerne des matrices artificielles pour contenir une séquence prédéterminée et une structure en épingle à cheveux par réaction d'extension d'oligonucléotides emboîtés. Cette matrice artificielle permet à l'amplification par amorce unique (SPA) d'amplifier une séquence cible dans la matrice artificielle. Dans des modes de réalisation particulièrement utiles, les séquences cibles provenant des matrices artificielles sont clonées dans des vecteurs d'expression de manière à obtenir une banque de polypeptides ou de protéines, telle que, par exemple, une banque d'anticorps.

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